T1192
Sigma
Monoclonal Anti-Thymine Dimer antibody produced in mouse
~2 mg/mL, clone H3, purified immunoglobulin, buffered aqueous solution
| MDL number: | MFCD01865339 |
Description
| Application | May be used in Southern blotting at 0.5-1μg/mL using UV induced thymine dimers of λ phage DNA fragments. |
| Features and Benefits | • Reacts specifically with the (5′-6′) cyclobutane type of thymine-thymine or thymine-cytosine heterodimers. • Allows detection of UV-photo induced thymine dimers in single-stranded DNA fragments greater than 20 residues in length. • Offers a sensitive and non-radioactive method for labeling, detecting, and quantitating specific DNA fragments using ELISA, immunocytochemistry and Southern blotting. • Enables analysis of high molecular weight DNA fragments when the degree of fragmentation is below the detection limit of conventional apoptosis-related staining techniques. |
| Immunogen | tetra nucleotide containing cyclobutane thymine dimer (GTTG) conjugated to chicken gamma globulin.1 |
| Legal Information | Distributed under license. Patents WO87/01134, EP 0233 177 B1 |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | purified immunoglobulin |
| clone | H3 (monoclonal) |
| form | buffered aqueous solution |
| species reactivity | chicken, wide range |
| concentration | ~2 mg/mL |
| application(s) | capture ELISA: suitable |
| immunocytochemistry: suitable | |
| isotype | IgG1 |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
References
| Cited Reference | 1. Roza, L., et al., Detection of Cyclobutane Thymine Dimers in DNA of Human Cells with Monoclonal Antibodies Raised Against a Thymine Dimer-Containing Tetranucleotide. Photochem. Photobiol. 48, 627-633, (1988) Abstract |
| reference | Marvik, O.J., et al., Photodetection: A Nonradioactive Labeling and Detection Method for DNA. Biotechniques 23, 892-896, (1997) Abstract |
| Slagsvold, H.H., et al., Detection of high molecular weight DNA fragments characteristic of early stage apoptosis in cerebellar granule cells exposed to glutamate. Exp. Brain Res. 135, 173-178, (2000) Abstract |
